Applicability of Environmental Microorganisms to Alkaline Single Cell Gel Electrophoresis (Comet) Assay.
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چکیده
منابع مشابه
Improvement of single-cell gel electrophoresis (SCGE) alkaline comet assay
Although the single-cell gel electrophoresis (SCGE) alkaline comet assay can detect DNA damage quickly and with high sensitivity, it does not work well for cryopreserved sperm of some marine teleosts. Using large yellow croaker Pseudosciaena crocea sperm that were cryopreserved in increasing concentrations (5 to 30%) of DMSO, we made modifications to the classic SCGE method that included using ...
متن کاملSingle Cell Gel Electrophoresis (Comet Assay) as a Tool for Environmental Biomonitoring. An Example of Pesticides
Methods that permit the sensitive detection of DNA damage and repair are of special significance in the field of environmental research due to the long latent period between exposure to environmental agent(s) and genetic effect(s) becoming apparent. Malathion is a commonly used organophosphorus insecticide reported to be genotoxic both in vivo and in vitro, but the reports are conflicting. In o...
متن کاملComparison of sensitivity to methyl methanesulphonate among tadpole developmental stages using the alkaline single-cell gel electrophoresis (comet) assay.
In a previous study, we demonstrated that tadpoles are suitable organisms for monitoring small bodies of water (e.g., creeks, ponds, and drainage ditches) for genotoxicity using the alkaline single-cell gel DNA electrophoresis (SCG) or "comet" assay [Ralph and Petras, 1997]. This approach involves detection, under alkaline conditions, of cell DNA fragments which on electrophoresis migrate from ...
متن کاملThe application of single cell gel electrophoresis or Comet assay to human monitoring studies.
OBJECTIVE In the search of new human genotoxic biomarkers, the single cell gel electrophoresis assay has been proposed as a sensible alternative. MATERIAL AND METHODS This technique detects principally single strand breaks as well as alkali-labile and repair-retarded sites. RESULTS Herein we present our experience using the single cell gel electrophoresis assay in human population studies, ...
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ژورنال
عنوان ژورنال: Japanese Journal of Water Treatment Biology
سال: 1999
ISSN: 0910-6758,1881-0438
DOI: 10.2521/jswtb.35.261